Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-c-Rel antibody [EPR2559(2)] (ab133251)

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Immunoprecipitation - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
  • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
  • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
  • Anti-c-Rel antibody [EPR2559(2)] (ab133251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2559(2)] to c-Rel
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-c-Rel antibody [EPR2559(2)]
    See all c-Rel primary antibodies

  • Description

    Rabbit monoclonal [EPR2559(2)] to c-Rel

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HuT-78, and HeLa cell lysates. IP: HeLa cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use at 1/10000 - 1/50000.
IP
1/10 - 1/100.
Notes
WB
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use at 1/10000 - 1/50000.
IP
1/10 - 1/100.

Target

  • Function

    Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.

  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Avian reticuloendotheliosis antibody
      • C REL antibody
      • C Rel protein antibody
      • c Rel proto oncogene protein antibody
      • Oncogene REL antibody
      • Oncogene REL avian reticuloendotheliosis antibody
      • Proto-oncogene c-Rel antibody
      • REL antibody
      • REL_HUMAN antibody
      • v rel avian reticuloendotheliosis viral oncogene homolog antibody
      • v rel reticuloendotheliosis viral oncogene homolog antibody
      • V rel reticuloendotheliosis viral oncogene homolog (avian) antibody
      see all

    Images

    • Immunoprecipitation - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Immunoprecipitation - Anti-c-Rel antibody [EPR2559(2)] (ab133251)

      Purified ab133251 at 1/20 dilution (0.6µg) immunoprecipitating c-Rel in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab133251 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133251 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 78 kDa
      Fresh lysate is required to avoid protein degradation.

    • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      All lanes : Anti-c-Rel antibody [EPR2559(2)] (ab133251) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa

    • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: c-Rel knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab133251 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133251 was shown to recognize c-Rel when c-Rel knockout samples were used, along with additional cross-reactive bands. Wild-type and c-Rel knockout samples were subjected to SDS-PAGE. ab133251 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Anti-c-Rel antibody [EPR2559(2)] (ab133251)

    References (1)

    Publishing research using ab133251? Please let us know so that we can cite the reference in this datasheet.

    ab133251 has been referenced in 1 publication.

    • Zhang C  et al. Activation of TNF-a/NF-?B axis enhances CRL4BDCAF11E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells. Mol Oncol N/A:N/A (2018). WB . PubMed: 29377600

    Customer reviews and Q&As

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    Western blot abreview for Anti-c-Rel antibody [EPR2559(2)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
    Read More

    Dr. Robert Kupp

    Verified customer

    Submitted Sep 17 2019

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