Overview

  • Product name

    Anti-c-Rel antibody [EPR2559(2)]
    See all c-Rel primary antibodies
  • Description

    Rabbit monoclonal [EPR2559(2)] to c-Rel
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human c-Rel was used as an immunogen (Q04864).

  • Positive control

    • Daudi and HuT-78 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
    • Sequence similarities

      Contains 1 RHD (Rel-like) domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Avian reticuloendotheliosis antibody
      • C REL antibody
      • C Rel protein antibody
      • c Rel proto oncogene protein antibody
      • Oncogene REL antibody
      • Oncogene REL avian reticuloendotheliosis antibody
      • Proto-oncogene c-Rel antibody
      • REL antibody
      • REL_HUMAN antibody
      • v rel avian reticuloendotheliosis viral oncogene homolog antibody
      • v rel reticuloendotheliosis viral oncogene homolog antibody
      • V rel reticuloendotheliosis viral oncogene homolog (avian) antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: c-Rel knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab133251 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133251 was shown to recognize c-Rel when c-Rel knockout samples were used, along with additional cross-reactive bands. Wild-type and c-Rel knockout samples were subjected to SDS-PAGE. ab133251 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-c-Rel antibody [EPR2559(2)] (ab133251) at 1/10000 dilution

      Lane 1 : Daudi cell lysates
      Lane 2 : HuT-78 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 68 kDa

    References

    This product has been referenced in:

    • Zhang C  et al. Activation of TNF-a/NF-?B axis enhances CRL4BDCAF11E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells. Mol Oncol N/A:N/A (2018). WB . Read more (PubMed: 29377600) »
    See 1 Publication for this product

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