Key features and details
- Rabbit polyclonal to c-Rel - N-terminal
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-c-Rel antibody - N-terminal
See all c-Rel primary antibodies
DescriptionRabbit polyclonal to c-Rel - N-terminal
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment within Human c-Rel (N terminal). The exact sequence is proprietary.
Database link: Q04864
- IP: Raji whole cell extract. IHC: Human skin tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|IHC-P||1/100 - 1/1000.|
FunctionProto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
- Information by UniProt
- Avian reticuloendotheliosis antibody
- C REL antibody
- C Rel protein antibody
c-Rel was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 5 μg ab227519. Western blot was performed from the immunoprecipitate using ab227519. Anti-rabbit IgG was used as secondary antibody.
Lane 1: Raji whole cell extract.
Lane 2: Control IgG IP instead of ab227519 in Raji whole cell extract.
Lane 3: ab227519 IP in Raji whole cell extract.
Paraffin-embedded human skin tissue stained for c-Rel using ab227519 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227519 has not yet been referenced specifically in any publications.