Product nameAnti-c-Rel (phospho S503) antibody
See all c-Rel primary antibodies
DescriptionRabbit polyclonal to c-Rel (phospho S503)
Specificityab30624 recognises endogenous levels of c-Rel only when phosphorylated at Serine 503.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.
- Breast carcinoma, MDA-MB-435 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab30624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 69 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionProto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
Sequence similaritiesContains 1 RHD (Rel-like) domain.
- Information by UniProt
- Avian reticuloendotheliosis antibody
- C REL antibody
- C Rel protein antibody
All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution
Lane 1 : extracts from
MDA-MB-435 cells (5-30ug).
Lane 2 : extracts from
MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).
Predicted band size: 69 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
ab30624 has not yet been referenced specifically in any publications.