Anti-c-Rel (phospho S503) antibody (ab30624)
Key features and details
- Rabbit polyclonal to c-Rel (phospho S503)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Rel (phospho S503) antibody
See all c-Rel primary antibodies -
Description
Rabbit polyclonal to c-Rel (phospho S503) -
Host species
Rabbit -
Specificity
ab30624 recognises endogenous levels of c-Rel only when phosphorylated at Serine 503. -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.
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Positive control
- Breast carcinoma, MDA-MB-435 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. -
Sequence similarities
Contains 1 RHD (Rel-like) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5966 Human
- Omim: 164910 Human
- SwissProt: Q04864 Human
- Unigene: 631886 Human
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Alternative names
- Avian reticuloendotheliosis antibody
- C REL antibody
- C Rel protein antibody
see all
Images
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All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution
Lane 1 : extracts from
MDA-MB-435 cells (5-30ug).
Lane 2 : extracts from
MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).
Predicted band size: 69 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Rel (phospho S503) antibody (ab30624)Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab30624 has not yet been referenced specifically in any publications.