Overview

  • Product name

    Anti-C10 antibody (Biotin)
    See all C10 primary antibodies
  • Description

    Rabbit polyclonal to C10 (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse C10.

  • General notes

    Protein target previously labeled as CCL6.

Properties

  • Form

    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab83134 is sterile filtered.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine CCL6.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant murine CCL6.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant murine CCL6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 13 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
    • Cellular localization

      Secreted
    • Database links

    • Alternative names

      • C10 antibody
      • CCL 6 antibody
      • chemokine (C-C motif) ligand 6 antibody
      • Chemokine C C motif Ligand 6 antibody
      • MRP1 antibody
      • SCYA6 antibody
      • Small Inducible Cytokine A6 antibody
      see all

    References

    ab83134 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your enquiry.

    The recombinant MurineCCL6 (C10) is also in our catalogue ab9898. This is the mature protein, which contains 95 amino acids (amino acids 22-116).

    The amino acid sequence is as follows:

    GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA

    The 21 amino acid N-terminal signal peptide is not present on the protein.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for your enquiry regarding ab83134.

    The immunogen used to raise this antibody was a highly pure (>98%) recombinant mouse CCL6. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognize a different epitope. For polyclonal antibodies epitope mapping experiment is not carried out.

    Currently, as our datasheet indicates we have no data with this product on cell/tissue samples yet this needs to be tested.

    If you need any further assistance or help, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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