Overview

  • Product name

    Anti-C10 antibody [EPR14614]
    See all C10 primary antibodies
  • Description

    Rabbit monoclonal [EPR14614] to C10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse C10 aa 1-100. The exact sequence is proprietary.
    Database link: P27784

  • Positive control

    • Recombinant mouse C10 protein, mouse and rat spleen tissues.
  • General notes

     

     

    Protein target previously labeled as CCL6.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14614
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • C10 antibody
    • CCL 6 antibody
    • chemokine (C-C motif) ligand 6 antibody
    • Chemokine C C motif Ligand 6 antibody
    • MRP1 antibody
    • SCYA6 antibody
    • Small Inducible Cytokine A6 antibody
    see all

Images

  • Anti-C10 antibody [EPR14614] (ab191400) at 1/10000 dilution + Recombinant mouse C10 protein at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

  • Immunohistochemical analysis of paraffin-embedded, mouse spleen tissue labeling C10 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling C10 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab191400 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191400.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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