RecombinantRabMAb

Recombinant Anti-C10 antibody [EPR14614] (ab191400)

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Western blot - Anti-C10 antibody [EPR14614] (ab191400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)
  • Anti-C10 antibody [EPR14614] (ab191400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14614] to C10
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-C10 antibody [EPR14614]
    See all C10 primary antibodies

  • Description

    Rabbit monoclonal [EPR14614] to C10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Mouse C10 aa 1-100. The exact sequence is proprietary.
    Database link: P27784

  • Positive control

    • Recombinant mouse C10 protein, mouse and rat spleen tissues.

  • General notes

     

     

    Protein target previously labeled as CCL6.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14614

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • C10 antibody
      • CCL 6 antibody
      • chemokine (C-C motif) ligand 6 antibody
      • Chemokine C C motif Ligand 6 antibody
      • MRP1 antibody
      • SCYA6 antibody
      • Small Inducible Cytokine A6 antibody
      see all

    Images

    • Western blot - Anti-C10 antibody [EPR14614] (ab191400)
      Western blot - Anti-C10 antibody [EPR14614] (ab191400)
      Anti-C10 antibody [EPR14614] (ab191400) at 1/10000 dilution + Recombinant mouse C10 protein at 20 µg

      Secondary
      goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 13 kDa
      Observed band size: 13 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)

      Immunohistochemical analysis of paraffin-embedded, mouse spleen tissue labeling C10 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)

      Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling C10 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-C10 antibody [EPR14614] (ab191400)
      Anti-C10 antibody [EPR14614] (ab191400)

    References (0)

    Publishing research using ab191400? Please let us know so that we can cite the reference in this datasheet.

    ab191400 has not yet been referenced specifically in any publications.

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