Overview

  • Product name

    Anti-C10orf35 antibody [EP14950-82]
    See all C10orf35 primary antibodies
  • Description

    Rabbit monoclonal [EP14950-82] to C10orf35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human C10orf35 aa 1-100. The exact sequence is proprietary.
    Database link: Q96D05

  • Positive control

    • Human fetal brain, fetal kidney, MCF7, A431, Mouse and rat brain lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP14950-82
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IP 1/50 - 1/70.

Target

Images

  • All lanes : Anti-C10orf35 antibody [EP14950-82] (ab189923) at 1/50000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : MCF7 lysate
    Lane 4 : A431 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

  • All lanes : Anti-C10orf35 antibody [EP14950-82] (ab189923) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

  • Western blot analysis of C10orf35 in Human fetal brain lysate immunoprecipitated using ab189923 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

References

ab189923 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189923.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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