Overview

  • Product name

    Anti-C10orf46/CAC1 antibody [EPR16094]
    See all C10orf46/CAC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16094] to C10orf46/CAC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human C10orf46/CAC1 aa 200-300. The exact sequence is proprietary.
    Database link: Q86Y37

  • Positive control

    • A375, HT1080, SW480 and Human breast cancer lysates; Human cervix squamous cell carcinoma and rat brain tissues; HeLa and A375 Cells.
  • General notes

     

     

     This product was previously labelled as C10orf46

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/40.
ICC/IF 1/100.

Target

  • Relevance

    Belongs to the cullin family. There are 3 isoforms.
  • Database links

  • Alternative names

    • Chromosome 10 open reading frame 46 antibody
    • Cullin antibody
    • FLJ40409 antibody
    • MGC33215 antibody
    • OTTHUMP00000046870 antibody
    • Uncharacterized protein C10orf46 homolog antibody
    see all

Images

  • All lanes : Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : Human breast cancer lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed A375 cells labeling C10orf46/CAC1 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling C10orf46/CAC1 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Western blot analysis of C10orf46/CAC1 in immunoprecipitation pellets from HT1080 lysate. ab190799 used at 1/40 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling C10orf46/CAC1 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

  • Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling C10orf46/CAC1 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

References

ab190799 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190799.
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