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  1. Link

    c11b2cyp11b2-antibody-epr10495-ab167413.pdf

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RecombinantRabMAb

Recombinant Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

  • Datasheet
  • SDS
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Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
  • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
  • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
  • Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
  • Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10495] to C11B2/CYP11B2
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-C11B2/CYP11B2 antibody [EPR10495]
    See all C11B2/CYP11B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10495] to C11B2/CYP11B2
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 5 consecutive identical amino acids with family members like CYP11B1, CYP3A4, CYP3A43, CYP3A5, CYP3A7. Cross-reactivity with these proteins have not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C11B2/CYP11B2 aa 300-400. The exact sequence is proprietary.
    Database link: P19099

  • Positive control

    • IP: MCF7 cell lysates WB: Human adrenal gland, NIH:OVCAR-3 and MCF7 cell lysates, human tissue lysates;
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10495
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism

Associated products

  • Alternative Versions

    • Anti-C11B2/CYP11B2 antibody [EPR10495] - BSA and Azide free (ab240955)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human C11B2/CYP11B2 protein (His tag) (ab224858)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone.
  • Involvement in disease

    Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency) [MIM:203400]; also known as aldosterone deficiency due to defect in 18-hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18-hydroxycorticosterone, is low or normal.
    Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency) [MIM:610600]. CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18-hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum.
    Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1) [MIM:103900]. It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. Note=The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Mitochondrion membrane.
  • Target information above from: UniProt accession P19099 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1585 Human
    • Omim: 124080 Human
    • SwissProt: P19099 Human
    • Unigene: 632054 Human
    • Alternative names

      • ALDOS antibody
      • Aldosterone synthase antibody
      • Aldosterone-synthesizing enzyme antibody
      • C11B2_HUMAN antibody
      • CYP11B2 antibody
      • CYPXIB2 antibody
      • Cytochrome P-450Aldo antibody
      • Cytochrome P-450C18 antibody
      • Cytochrome P450 11B2 antibody
      • Cytochrome P450 11B2, mitochondrial antibody
      • mitochondrial antibody
      • P-450Aldo antibody
      • P-450C18 antibody
      • Steroid 18-hydroxylase antibody
      see all

    Images

    • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

      Lane 1 : Human adrenal gland tissue lysate
      Lane 2 : Human kidney tissue lysate
      Lane 3 : Human heart tissue lysate
      Lane 4 : Human lung tissue lysate
      Lane 5 : Human liver tissue lysate
      Lane 6 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 7 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 58 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate with Human C11B2/CYP11B2 peptide
      Lane 2 : MCF-7 cell lysate with Human C11B1/CYP11B2 peptide
      Lane 3 : MCF-7 cell lysate with no blocking peptide

      Predicted band size: 58 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds
    • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

      Lane 1 : Human adrenal gland tissue lysate
      Lane 2 : NIH:OVCAR-3 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

      Detection of C11B2/CYP11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.

    • Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab167413? Please let us know so that we can cite the reference in this datasheet.

    ab167413 has been referenced in 3 publications.

    • Stelcer E  et al. Adropin Stimulates Proliferation and Inhibits Adrenocortical Steroidogenesis in the Human Adrenal Carcinoma (HAC15) Cell Line. Front Endocrinol (Lausanne) 11:561370 (2020). PubMed: 33133015
    • Zhang G  et al. MiR-193a-3p functions as a tumour suppressor in human aldosterone-producing adrenocortical adenoma by down-regulating CYP11B2. Int J Exp Pathol 99:77-86 (2018). PubMed: 29665181
    • França MM  et al. POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells. Braz J Med Biol Res 48:1087-94 (2015). WB . PubMed: 26421867

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