Recombinant
RabMAb

Recombinant Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

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Overview

  • Product name

    Anti-C11B2/CYP11B2 antibody [EPR10495]
    See all C11B2/CYP11B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10495] to C11B2/CYP11B2

  • Host species

    Rabbit

  • Specificity

    In house testing has shown no cross-reactivity with C11B1 in either western blot (see image) or peptide ELISA.

  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human C11B2/CYP11B2 aa 300-400. The exact sequence is proprietary.
    Database link: P19099

  • Positive control

    • Human adrenal gland, NIH:OVCAR-3 and MCF7 cell lysates; Human heart and Human kidney tissues.

  • General notes

     

     

     This product was previously labelled as C11B2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab167413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone.

    • Involvement in disease

      Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency) [MIM:203400]; also known as aldosterone deficiency due to defect in 18-hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18-hydroxycorticosterone, is low or normal.
      Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency) [MIM:610600]. CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18-hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum.
      Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1) [MIM:103900]. It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. Note=The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2.

    • Sequence similarities

      Belongs to the cytochrome P450 family.

    • Cellular localization

      Mitochondrion membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ALDOS antibody
      • Aldosterone synthase antibody
      • Aldosterone-synthesizing enzyme antibody
      • C11B2_HUMAN antibody
      • CYP11B2 antibody
      • CYPXIB2 antibody
      • Cytochrome P-450Aldo antibody
      • Cytochrome P-450C18 antibody
      • Cytochrome P450 11B2 antibody
      • Cytochrome P450 11B2, mitochondrial antibody
      • mitochondrial antibody
      • P-450Aldo antibody
      • P-450C18 antibody
      • Steroid 18-hydroxylase antibody
      see all

    Images

    • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate with Human C11B2/CYP11B2 peptide
      Lane 2 : MCF-7 cell lysate with Human C11B1/CYP11B2 peptide
      Lane 3 : MCF-7 cell lysate with no blocking peptide

      Predicted band size: 58 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 seconds
    • Flow Cytometry - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Flow Cytometry - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Overlay histogram showing MCF7 cells stained with ab167413 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab167413, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      All lanes : Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

      Lane 1 : Human adrenal gland tissue lysate
      Lane 2 : NIH:OVCAR-3 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

      Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C11B2/CYP11B2 with ab167413 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C11B2/CYP11B2 with ab167413 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)
      Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

      Detection of C11B2/CYP11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.

    References

    Publishing research using ab167413? Please let us know so that we can cite the reference in this datasheet.

    ab167413 has been referenced in 2 publications.

    • Zhang G  et al. MiR-193a-3p functions as a tumour suppressor in human aldosterone-producing adrenocortical adenoma by down-regulating CYP11B2. Int J Exp Pathol 99:77-86 (2018). PubMed: 29665181
    • França MM  et al. POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells. Braz J Med Biol Res 48:1087-94 (2015). WB . PubMed: 26421867

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C11B2/CYP11B2 antibody [EPR10495]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Baboon Tissue sections (adrenal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0
    Permeabilization
    Yes - 0.4% triton x-100 in KPBS
    Specification
    adrenal
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

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    Submitted Mar 06 2020

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