Overview

  • Product name

  • Description

    Rabbit polyclonal to C11orf17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human C11orf17.

  • Positive control

    • Lysates from HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab135996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    C11orf17 is a nuclear protein and known to bind to the amino terminus (residues 1-39) of the C subunit of PKA. It is a binding partner of NF-kappaB p65 subunit, and enhances NF-kappaB-mediated gene expression.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AKIP1 antibody
    • BCA3 antibody
    • Breast cancer associated gene 3 protein antibody
    • Chromosome 11 open reading frame 17 antibody
    • Koyt binding protein antibody
    • PKA interacting protein antibody
    • Proline rich protein BCA3 antibody
    • Protein kinase A interacting protein 1 antibody
    see all

Images

  • Anti-C11orf17 antibody (ab135996) at 1/500 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 23 kDa

References

ab135996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Hela and HepG2)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Hela and HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
No
Specification
Hela
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Dr. Lin Zhu

Verified customer

Submitted Sep 07 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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