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Rabbit polyclonal to C11orf45
antigen sequence: SSAVYTHGCG CVRSATNITC QSSGQQRQAA RQEEENSICK AHDSREGRLG YPLSAHQPGS GGPN, corresponding to C terminal amino acids 82-145 of Human C11orf45.
- Human breast tissue; C11orf45 over-expression HEK293T lysate.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- C11orf45 antibody
- Chromosome 11 open reading frame 45 antibody
- CK045_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-C11orf45 antibody (ab122577)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122577 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C11orf45 antibody (ab122577)
ab122577, at 1/50 dilution, staining C11orf45 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Western blot - Anti-C11orf45 antibody (ab122577)
All lanes : Anti-C11orf45 antibody (ab122577) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : C11orf45 over-expression HEK293T Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"