Overview

  • Product name
    Anti-C11orf45 antibody
  • Description
    Rabbit polyclonal to C11orf45
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SSAVYTHGCG CVRSATNITC QSSGQQRQAA RQEEENSICK AHDSREGRLG YPLSAHQPGS GGPN, corresponding to C terminal amino acids 82-145 of Human C11orf45.

  • Positive control
    • Human breast tissue; C11orf45 over-expression HEK293T lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122577 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122577, at 1/50 dilution, staining C11orf45 in paraffin-embedded Human breast tissue by Immunohistochemistry.
  • All lanes : Anti-C11orf45 antibody (ab122577) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)

    Lane 2 : C11orf45 over-expression HEK293T Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

    Developed using the ECL technique.

References

ab122577 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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