Overview

  • Product name
    Anti-C11orf95 antibody - C-terminal
  • Description
    Rabbit polyclonal to C11orf95 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C11orf95 aa 563-592 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: C9JLR9

  • Positive control
    • Human adipose tissue; HepG2 cells and lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170283 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 73 kDa.
ICC/IF 1/10 - 1/50.

Target

Images

  • Confocal immunofluorescent analysis of HepG2 cells labeling C11orf95 with ab170283 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adipose tissue labeling C11orf95 with ab170283 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

  • Anti-C11orf95 antibody - C-terminal (ab170283) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 73 kDa

References

ab170283 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Robert Kupp

Verified customer

Submitted Jun 04 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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