Product nameC12 E8 solution, Non-ionic detergent
- Dodecyl octaethylene glycol ether
Non-ionic detergent used for solubilization of membrane-bound proteins in their native state. Used in a mixed micellar assay for lipoxygenase acting at neutral pH and for functional reconstitution of influenza virus envelopes.
Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSupplied as a 10% (w/v) aqueous solution
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Fogeron ML et al. Wheat germ cell-free expression: Two detergents with a low critical micelle concentration allow for production of soluble HCV membrane proteins. Protein Expr Purif 105:39-46 (2015). Read more (PubMed: 25306874) »
- Romero F Solubilization and partial characterization of ouabain-insensitive Na(+)-ATPase from basolateral plasma membranes of the intestinal epithelial cells. Invest Clin 50:303-14 (2009). Read more (PubMed: 19961053) »
- Musatov A et al. Detergent-solubilized bovine cytochrome c oxidase: dimerization depends on the amphiphilic environment. Biochemistry 39:12996-3004 (2000). Read more (PubMed: 11041865) »
- López-Nicolás JM et al. An octaethylene glycol monododecyl ether-based mixed micellar assay for lipoxygenase acting at neutral pH. Anal Biochem 221:410-5 (1994). Read more (PubMed: 7810887) »
- Levy D et al. Phospholipid vesicle solubilization and reconstitution by detergents. Symmetrical analysis of the two processes using octaethylene glycol mono-n-dodecyl ether. Biochemistry 29:9480-8 (1990). Read more (PubMed: 2248960) »
- Stegmann T et al. Functional reconstitution of influenza virus envelopes. EMBO J 6:2651-9 (1987). Read more (PubMed: 3678202) »