Anti-C12orf34 antibody (ab122626)
Key features and details
- Rabbit polyclonal to C12orf34
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C12orf34 antibody -
Description
Rabbit polyclonal to C12orf34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PSIHSLLYQL NQQCQAPGAA PPACQGMAIP HPSPAKHGPV PSFPSMAYSA AAGLPDCRKG TELGQGATQA LTLAGAAKPA GYADSGLDYL LWPQKP, corresponding to amino acids 197-292 of Human C12orf34.
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Positive control
- IHC-P: Human stomach, testis, and cerebral cortex tissues; ICC: U 251 MG cells; WB: SH-SY5Y whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 84915 Human
- SwissProt: Q5U5X8 Human
- Unigene: 661785 Human
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Alternative names
- C12orf34 antibody
- Chromosome 12 open reading frame 34 antibody
- CL034_HUMAN antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Anti-C12orf34 antibody (ab122626) + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of stomach tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of cerebral cortex tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of testis tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of skeletal muscle tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity in myocytes as expected.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab122626 has been referenced in 2 publications.
- Yan T et al. FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease. Nat Commun 11:411 (2020). PubMed: 31964863
- Satoh JI et al. Reactive astrocytes express Aggregatin (FAM222A) in the brains of Alzheimer's disease and Nasu-Hakola disease. Intractable Rare Dis Res 9:217-221 (2020). PubMed: 33139980