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Rabbit polyclonal to C12orf34
antigen sequence: PSIHSLLYQL NQQCQAPGAA PPACQGMAIP HPSPAKHGPV PSFPSMAYSA AAGLPDCRKG TELGQGATQA LTLAGAAKPA GYADSGLDYL LWPQKP, corresponding to amino acids 197-292 of Human C12orf34.
Human stomach tssue; U 251 MG lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
Chromosome 12 open reading frame 34 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (ab122626)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)
ab122626 at 1/200 dilution staining C12orf34 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
Western blot - Anti-C12orf34 antibody (ab122626)
All lanes : Anti-C12orf34 antibody (ab122626) at 1/250 dilution Lane 1 : RT 4 lysate Lane 2 : U 251 MG lysate Lane 3 : Human plasma lysate Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique.
has not yet been referenced specifically in any publications.
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