Overview

  • Product name

    Anti-C12orf41 antibody
  • Description

    Rabbit polyclonal to C12orf41
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 103-180 of Human C12orf41.

  • Positive control

    • Human pancreas tissue; RT4 and U 251 MG cell lysates; Human Liver and Tonsil lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 55 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab150848 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling C12orf41 with ab150848 at 1/500 dilution.
  • All lanes : Anti-C12orf41 antibody (ab150848) at 1/100 dilution

    Lane 1 : RT4
    cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Predicted band size: 55 kDa

References

ab150848 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150848.
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