Anti-C13orf18 antibody (ab246954)
Key features and details
- Rabbit polyclonal to C13orf18
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C13orf18 antibody -
Description
Rabbit polyclonal to C13orf18 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human C13orf18 aa 180-313.
Sequence:AVQVSRRTISSNSFSPEVFVLPVDVEKENAHFYVADMIISAMEKMKCNIL SQQQTESWSKEVSGLLGSDQPDSEMTFDTNIKQESGSSTSSYSGYEGCAV LQVSPVTETRTYHDVKEICKCDVDEFVILELGDF
Database link: Q9H714 -
Positive control
- IHC-P: Human duodenum tissue. WB: RT4 and U-251 MG cell lysate; Human liver and tonsil tissue lysate. ICC/IF: U-251 MG cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab246954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 80183 Human
- SwissProt: Q9H714 Human
- Unigene: 98117 Human
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Alternative names
- C13orf18 antibody
- CM018_HUMAN antibody
- FLJ21562 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-C13orf18 antibody (ab246954)
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling C13orf18 using ab246954 at 4 µg/ml (green) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C13orf18 antibody (ab246954)Paraffin-embedded human duodenum tissue stained for C13orf18 using ab246954 at 1/50 dilution in immunohistochemical analysis. -
Western blot - Anti-C13orf18 antibody (ab246954)All lanes : Anti-C13orf18 antibody (ab246954) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab246954 has not yet been referenced specifically in any publications.
