Anti-C13orf18 antibody (ab246954)

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Overview

  • Product name

    Anti-C13orf18 antibody
    See all C13orf18 primary antibodies

  • Description

    Rabbit polyclonal to C13orf18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human C13orf18 aa 180-313.
    Sequence:

    AVQVSRRTISSNSFSPEVFVLPVDVEKENAHFYVADMIISAMEKMKCNIL SQQQTESWSKEVSGLLGSDQPDSEMTFDTNIKQESGSSTSSYSGYEGCAV LQVSPVTETRTYHDVKEICKCDVDEFVILELGDF


    Database link: Q9H714

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human duodenum tissue. WB: RT4 and U-251 MG cell lysate; Human liver and tonsil tissue lysate. ICC/IF: U-251 MG cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-C13orf18 antibody (ab246954)
    Immunocytochemistry/ Immunofluorescence - Anti-C13orf18 antibody (ab246954)

    PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling C13orf18 using ab246954 at 4 µg/ml (green) in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C13orf18 antibody (ab246954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C13orf18 antibody (ab246954)
    Paraffin-embedded human duodenum tissue stained for C13orf18 using ab246954 at 1/50 dilution in immunohistochemical analysis.
  • Western blot - Anti-C13orf18 antibody (ab246954)
    Western blot - Anti-C13orf18 antibody (ab246954)
    All lanes : Anti-C13orf18 antibody (ab246954) at 0.4 µg/ml

    Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
    Lane 3 : Human plasma (IgG/HSA depleted)
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

References

ab246954 has not yet been referenced specifically in any publications.

Publishing research using ab246954? Please let us know so that we can cite the reference in this datasheet.

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