Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionRequired for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Sequence similaritiesContains 1 C2H2-type zinc finger.
modificationsPhosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
Cellular localizationNucleus. Chromosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
- C13orf8 antibody
- CAMP antibody
- CHAMP antibody
All lanes : Anti-C13orf8 antibody (ab169249) at 1 µg/ml
Lane 1 : C13orf8 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 89 kDa
Immunofluorescent analysis of HeLa cells labeling C13orf8 with ab169249 at 10 µg/ml.
ab169249 has not yet been referenced specifically in any publications.