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    c14orf133-antibody-ab125084.pdf

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Anti-C14orf133 antibody (ab125084)

  • Datasheet
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Abpromise

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Western blot - Anti-C14orf133 antibody (ab125084)
  • Immunoprecipitation - Anti-C14orf133 antibody (ab125084)

Key features and details

  • Rabbit polyclonal to C14orf133
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-C14orf133 antibody
    See all C14orf133 primary antibodies
  • Description

    Rabbit polyclonal to C14orf133
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 443-493 of Human C14orf133 (NP_071350.2).

  • Positive control

    • HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab125084 was affinity purified using an epitope specific to C14orf133 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab125084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Plays a role in lysosomal trafficking. May play a role in epithelial polarization through stabilization of apical membrane protein content, possibly via the RAB11A-dependent apical recycling pathway. Also involved in direct or indirect transcriptional regulation of E-cadherin.
  • Involvement in disease

    Defects in VIPAR are the cause of arthrogryposis-renal dysfunction-cholestasis syndrome type 2 (ARCS2) [MIM:613404]. ARCS2 is an autosomal recessive multisystem disorder, characterized by neurogenic arthrogryposis multiplex congenita, renal tubular dysfunction and neonatal cholestasis with bile duct hypoplasia and low gamma glutamyl transpeptidase activity. Platelet dysfunction is common. Note=In liver, CEACAM5 and ABCB11 are mislocalized and E-cadherin expression is decreased.
  • Sequence similarities

    Belongs to the VIPAR family.
  • Cellular localization

    Early endosome. Recycling endosome. Late endosome.
  • Target information above from: UniProt accession Q9H9C1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 63894 Human
    • Entrez Gene: 104799 Mouse
    • Omim: 613401 Human
    • SwissProt: Q9H9C1 Human
    • SwissProt: Q8BGQ1 Mouse
    • Unigene: 16157 Human
    • Unigene: 208750 Mouse
    • Alternative names

      • Apical basolateral polarity regulator antibody
      • FLJ12707 antibody
      • hSPE-39 antibody
      • Protein spe-39 homolog antibody
      • SPE 39 antibody
      • SPE 39 protein antibody
      • SPE39 antibody
      • Uncharacterized protein C14orf133 antibody
      • vipar antibody
      • VIPAR_HUMAN antibody
      • VPS16B antibody
      • VPS33B interacting protein antibody
      • VPS33B interacting protein apical basolateral polarity regulator antibody
      • VPS33B interacting protein involved in polarity and apical protein restriction antibody
      • VPS33B-interacting protein antibody
      see all

    Images

    • Western blot - Anti-C14orf133 antibody (ab125084)
      Western blot - Anti-C14orf133 antibody (ab125084)
      All lanes : Anti-C14orf133 antibody (ab125084) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 57 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-C14orf133 antibody (ab125084)
      Immunoprecipitation - Anti-C14orf133 antibody (ab125084)
      Detection of C14orf133 by Western Blot of Immunprecipitate.
      ab125084 at 1µg/ml staining C14orf133 in HeLa whole cell lysate immunoprecipitated using ab125084 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab125084? Please let us know so that we can cite the reference in this datasheet.

    ab125084 has not yet been referenced specifically in any publications.

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