Anti-C14orf133 antibody (ab125084)
Key features and details
- Rabbit polyclonal to C14orf133
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-C14orf133 antibody
See all C14orf133 primary antibodies -
Description
Rabbit polyclonal to C14orf133 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 443-493 of Human C14orf133 (NP_071350.2).
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Positive control
- HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab125084 was affinity purified using an epitope specific to C14orf133 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab125084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 57 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Plays a role in lysosomal trafficking. May play a role in epithelial polarization through stabilization of apical membrane protein content, possibly via the RAB11A-dependent apical recycling pathway. Also involved in direct or indirect transcriptional regulation of E-cadherin. -
Involvement in disease
Defects in VIPAR are the cause of arthrogryposis-renal dysfunction-cholestasis syndrome type 2 (ARCS2) [MIM:613404]. ARCS2 is an autosomal recessive multisystem disorder, characterized by neurogenic arthrogryposis multiplex congenita, renal tubular dysfunction and neonatal cholestasis with bile duct hypoplasia and low gamma glutamyl transpeptidase activity. Platelet dysfunction is common. Note=In liver, CEACAM5 and ABCB11 are mislocalized and E-cadherin expression is decreased. -
Sequence similarities
Belongs to the VIPAR family. -
Cellular localization
Early endosome. Recycling endosome. Late endosome. - Information by UniProt
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Database links
- Entrez Gene: 63894 Human
- Entrez Gene: 104799 Mouse
- Omim: 613401 Human
- SwissProt: Q9H9C1 Human
- SwissProt: Q8BGQ1 Mouse
- Unigene: 16157 Human
- Unigene: 208750 Mouse
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Alternative names
- Apical basolateral polarity regulator antibody
- FLJ12707 antibody
- hSPE-39 antibody
see all
Images
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Western blot - Anti-C14orf133 antibody (ab125084)All lanes : Anti-C14orf133 antibody (ab125084) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-C14orf133 antibody (ab125084)Detection of C14orf133 by Western Blot of Immunprecipitate.
ab125084 at 1µg/ml staining C14orf133 in HeLa whole cell lysate immunoprecipitated using ab125084 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
References (0)
ab125084 has not yet been referenced specifically in any publications.
