Key features and details
- Rabbit polyclonal to C14orf169 / NO66
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C14orf169 / NO66 antibody
See all C14orf169 / NO66 primary antibodies
DescriptionRabbit polyclonal to C14orf169 / NO66
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla
Synthetic peptide corresponding to Human C14orf169/ NO66 aa 50-100.
Database link: Q9H6W3
- HeLa, 293T and Jurkat whole cell lysates
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab113975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 71 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHistone demethylase that specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region.
Sequence similaritiesBelongs to the MINA53/NO66 family. NO66 subfamily.
Contains 1 JmjC domain.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.
- Information by UniProt
- 60S ribosomal protein L8 histidine hydroxylase antibody
- C14orf169 antibody
- Chromosome 14 open reading frame 169 antibody
All lanes : Anti-C14orf169 / NO66 antibody (ab113975) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 30 seconds
Detection of C14orf169 / NO66 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113975 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab113975 has been referenced in 1 publication.
- Bräuer KE et al. Phylogenetic and genomic analyses of the ribosomal oxygenases Riox1 (No66) and Riox2 (Mina53) provide new insights into their evolution. BMC Evol Biol 18:96 (2018). PubMed: 29914368