Anti-C15orf40 antibody (ab243492)
Key features and details
- Rabbit polyclonal to C15orf40
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C15orf40 antibody -
Description
Rabbit polyclonal to C15orf40 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human C15orf40 aa 87-146.
Sequence:NVAIAAPPSEGEANAELCRYLSKVLELRKSDVVLDKGGKSREKVVKLLAS TTPEEILEKL
Database link: Q8WUR7 -
Positive control
- IHC-P: Human kidney tissue. WB: C15orf40 over-expression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag). ICC/IF: PC-3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab243492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Sequence similarities
Belongs to the UPF0235 family. - Information by UniProt
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Database links
- Entrez Gene: 123207 Human
- SwissProt: Q3ZBP8 Cow
- SwissProt: Q8WUR7 Human
- SwissProt: Q9CRC3 Mouse
- SwissProt: Q505I4 Rat
- Unigene: 352541 Human
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Alternative names
- C15orf40 antibody
- Chromosome 15 open reading frame 40 antibody
- CO040_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C15orf40 antibody (ab243492)
Paraffin-embedded human kidney tissue stained for C15orf40 using ab243492 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-C15orf40 antibody (ab243492)All lanes : Anti-C15orf40 antibody (ab243492) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : C15orf40 over-expression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 16 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-C15orf40 antibody (ab243492)PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for C15orf40 (green) using ab243492 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab243492 has not yet been referenced specifically in any publications.
