Recombinant
RabMAb

Recombinant Anti-C15orf41 antibody [EPR21169-28] (ab215190)

Overview

  • Product name

    Anti-C15orf41 antibody [EPR21169-28]
    See all C15orf41 primary antibodies
  • Description

    Rabbit monoclonal [EPR21169-28] to C15orf41
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant fragment within Human C15orf41 aa 1-200. The exact sequence is proprietary.
    Database link: Q9Y2V0

  • Positive control

    • WB: HEK-293T, RAW 264.7, PC-12, NIH/3T3 and HeLa whole cell lysate. Mouse spleen and kidney lysate. IP: HEK-293 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21169-28
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    Images

    • All lanes : Anti-C15orf41 antibody [EPR21169-28] (ab215190) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : C15orf41 siRNA knockdown HEK-293T whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa


      Exposure time: 3 minutes


      siRNA control and C15orf41 knockdown lysates were provided by an anonymous collaborator.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-C15orf41 antibody [EPR21169-28] (ab215190) at 1/1000 dilution

      Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Mouse spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • C15orf41 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab215190 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215190 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

      Lane 1: HEK-293 whole cell lysate 10 µg (Input).
      Lane 2: ab215190 IP in HEK-293 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215190 in HEK-293 whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 3 minutes.

    References

    ab215190 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab215190.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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