Overview

  • Product name

  • Description

    Rabbit polyclonal to C16orf48
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human C16orf48 aa 34-105.
    Sequence:

    EGNALKLDLLTSDRALDTTAPRGPCIGPGAGEILERGQRGVGDVLLQLEG ISLGPGASLKRKDPKDHEKENL


    Database link: Q9H0I2

  • Positive control

    • IHC-P: Human thyroid gland tissue. WB: C16orf48 overexpression HEK-293T cell lysate. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 38 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Sequence similarities

    Contains 1 enkurin domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chromosome 16 open reading frame 48 antibody
    • DAKV6410 antibody
    • DKFZp434A1319 antibody
    • Enkd1 antibody
    • ENKD1_HUMAN antibody
    • Enkurin domain containing 1 antibody
    • Enkurin domain-containing protein 1 antibody
    • Hypothetical protein LOC84080 antibody
    • UNQ6410 antibody
    see all

Images

  • Paraffin-embedded human thyroid gland tissue stained for C16orf48 using ab224560 at 1/200 dilution in immunohistochemical analysis.

  • All lanes : Anti-C16orf48 antibody (ab224560) at 1/100 dilution

    Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : C16orf48 overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

    Developed using the ECL technique.

    Predicted band size: 38 kDa

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for C16orf48 (green) using ab224560 at 4 µg/ml in ICC/IF.

References

ab224560 has not yet been referenced specifically in any publications.

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