Anti-C16orf62 antibody (ab97889)
Key features and details
- Rabbit polyclonal to C16orf62
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C16orf62 antibody
See all C16orf62 primary antibodies -
Description
Rabbit polyclonal to C16orf62 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-138 of Human C16orf62.
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Positive control
- 293T cell lysate, A431 cell lysate, H1299 cell lysate, HeLaS3 cell lysate, Molt-4 cell lysate, Raji cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 110 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes. Seems not to be required for CCC complex stability (PubMed:25355947). -
Sequence similarities
Belongs to the UPF0505 family. -
Cellular localization
Membrane. Early endosome. - Information by UniProt
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Database links
- Entrez Gene: 57020 Human
- Entrez Gene: 71517 Mouse
- Entrez Gene: 361635 Rat
- SwissProt: Q7Z3J2 Human
- SwissProt: Q8BWQ6 Mouse
- SwissProt: Q5XI83 Rat
- Unigene: 654964 Human
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Alternative names
- C16orf62 antibody
- Chromosome 16 open reading frame 62 antibody
- CP062_HUMAN antibody
see all
Images
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Western blot - Anti-C16orf62 antibody (ab97889)All lanes : Anti-C16orf62 antibody (ab97889) at 1/1000 dilution
Lane 1 : Molt4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C16orf62 antibody (ab97889)ab97889, at 1/500 dilution, staining C16orf62 by immunohistochemistry (parafin embedded sections), in Cal27 xenograft.
Protocols
References (1)
ab97889 has been referenced in 1 publication.
- McNally KE et al. Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. Nat Cell Biol 19:1214-1225 (2017). WB . PubMed: 28892079
