Overview

  • Product name

    Anti-C17orf27 antibody
  • Description

    Rabbit polyclonal to C17orf27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human C17orf27 aa 202-350.
    Database link: Q63HN8

  • Positive control

    • IHC-P: Human gastric cancer and colon cancer tissues. ICC/IF: A549 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/200 - 1/500.

Target

  • Involvement in disease

    Note=A chromosomal aberration involving ALO17 is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALK.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALK lymphoma oligomerization partner on chromosome 17 antibody
    • ALO17_HUMAN antibody
    • C17orf27 antibody
    • Chromosome 17 open reading frame 27 antibody
    • E3 ubiquitin-protein ligase RNF213 antibody
    • FLJ13051 antibody
    • KIAA1554 antibody
    • KIAA1618 antibody
    • MGC46622 antibody
    • MGC9929 antibody
    • MYMY2 antibody
    • Mysterin antibody
    • MYSTR antibody
    • NET57 antibody
    • Protein ALO17 antibody
    • RING finger protein 213 antibody
    • RNF 213 antibody
    • RNF213 antibody
    see all

Images

  • Paraffin-embedded human colon cancer tissue stained for RNF213 using ab238677 at 1/800 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human gastric cancer tissue stained for RNF213 using ab238677 at 1/800 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • A549 (human lung carcinoma cell line) cells stained for RNF213 (green) using ab238677 at 1/266 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear counterstain was DAPI (Blue).

References

ab238677 has not yet been referenced specifically in any publications.

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