Key features and details
- Rabbit polyclonal to C17orf71
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C17orf71 antibody
DescriptionRabbit polyclonal to C17orf71
Tested applicationsSuitable for: IHC-P, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
Synthetic peptide corresponding a region between residues 250 and 300 of human C17orf71 (NP_060619.4)
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to C17orf71 immobilized on solid support.
Our Abpromise guarantee covers the use of ab80472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceC17orf71 belongs to the UPF0487 family. There are 3 isoforms produced by alternative splicing.
- ABC 2 antibody
- ABC2 antibody
- Amplified in breast cancer gene 2 protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling C17orf71 with ab80472 at 1/1000 (1µg/ml). Detection: DAB.
Detection of human C17orf71 by Western Blot of Immunoprecipitates in whole cell lysates (1 mg for IP, 20% of IP loaded) from HeLa cells (lane 1), using 3 µg/mg of lysate of ab80472 for IP.
Lane 2: Control IgG.
For blotting immunoprecipitated C17orf71, ab80472 was used at 1 µg/ml.
Detection: via Chemiluminescence with an exposure time of 10 seconds.
ab80472 has not yet been referenced specifically in any publications.