Overview

  • Product name
  • Description
    Rabbit polyclonal to C17orf71
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    Synthetic peptide corresponding a region between residues 250 and 300 of human C17orf71 (NP_060619.4)

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was affinity purified using an epitope specific to C17orf71 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      C17orf71 belongs to the UPF0487 family. There are 3 isoforms produced by alternative splicing.
    • Database links
    • Alternative names
      • ABC 2 antibody
      • ABC2 antibody
      • Amplified in breast cancer gene 2 protein antibody
      • Chromosome 17 open reading antibody
      • chromosome 17 open reading frame 71 antibody
      • FLJ10587 antibody
      • Protein smg 8 homolog antibody
      • protein SMG8 antibody
      • SMG8 antibody
      • SMG8 protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling C17orf71 with ab80472 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of human C17orf71 by Western Blot of Immunoprecipitates in whole cell lysates (1 mg for IP, 20% of IP loaded) from HeLa cells (lane 1), using 3 µg/mg of lysate of ab80472 for IP.
      Lane 2: Control IgG.
      For blotting immunoprecipitated C17orf71, ab80472 was used at 1 µg/ml.
      Detection: via Chemiluminescence with an exposure time of 10 seconds.

    References

    ab80472 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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