Overview

  • Product name

    Anti-C18orf32 antibody
  • Description

    Rabbit polyclonal to C18orf32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: PFVSRIWPKK AIQESNDTNK GKVNFKGADM NGLPTKGPTE ICDK, corresponding to amino acids 30-73 of Human C18orf32.

  • Positive control

    • Human cerebellum tissue; RT 4, U 251 MG and Human liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    May activate the NF-kappa-B signaling pathway.
  • Sequence similarities

    Belongs to the UPF0729 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • C18orf32 antibody
    • Chromosome 18 open reading frame 32 antibody
    • CR032_HUMAN antibody
    • FLJ23458 antibody
    • Putative NF-kappa-B-activating protein 200 antibody
    • UPF0729 protein C18orf32 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122677 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122677, at 1/20 dilution, staining C18orf32 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • All lanes : Anti-C18orf32 antibody (ab122677) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References

This product has been referenced in:

  • Bersuker K  et al. A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Dev Cell 44:97-112.e7 (2018). Read more (PubMed: 29275994) »
See 1 Publication for this product

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