Overview

  • Product name

  • Description

    Rabbit polyclonal to C19orf2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human C19orf2 aa 180-298.
    Database link: O94763

  • Positive control

    • IHC-P: Human salivary gland and adrenal gland tissues. WB: U-87 MG whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/300.

Target

  • Function

    Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.
    Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival sigaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity at mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.
  • Tissue specificity

    Ubiquitous. Expressed in ovarian cancers (at protein level). Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells.
  • Sequence similarities

    Belongs to the RNA polymerase II subunit 5-mediating protein family.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation occurs essentially on serine residues. Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-372 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-372 disrupts the URI1-PPP1CC complex in the mitochondrion, releaves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity and preventing sustained S6K1-dependent signaling.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Cell projection > dendrite. Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 at mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • C19orf2 antibody
    • Chromosome 19 open reading frame 2 antibody
    • NNX3 antibody
    • NNX3 protein antibody
    • PPP1R19 antibody
    • Protein NNX3 antibody
    • Protein phosphatase 1 regulatory subunit 19 antibody
    • RMP antibody
    • RMP_HUMAN antibody
    • RNA polymerase II subunit 5 mediating protein antibody
    • RNA polymerase II subunit 5-mediating protein antibody
    • RPB5 mediating protein antibody
    • RPB5-mediating protein antibody
    • Unconventional prefoldin RPB5 interactor 1 antibody
    • Unconventional prefoldin RPB5 interactor antibody
    • URI antibody
    • URI1 antibody
    • URI1, prefoldin-like chaperone antibody
    see all

Images

  • Anti-C19orf2 antibody (ab235398) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 60 kDa

  • Paraffin-embedded human salivary gland tissue stained for C19orf2 using ab235398 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Paraffin-embedded human adrenal gland tissue stained for C19orf2 using ab235398 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

References

ab235398 has not yet been referenced specifically in any publications.

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