Overview

  • Product name

    Anti-C19orf2 antibody [SP215] - C-terminal
    See all C19orf2 primary antibodies
  • Description

    Rabbit monoclonal [SP215] to C19orf2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human C19orf2 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O94763

  • Positive control

    • Human 293 cells. Human ovarian adenocarcinoma tissue FC: 293T and C6 cells
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP215
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform Antigen Retrieval by boiling tissue sections in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.
    Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival sigaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity at mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.
  • Tissue specificity

    Ubiquitous. Expressed in ovarian cancers (at protein level). Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells.
  • Sequence similarities

    Belongs to the RNA polymerase II subunit 5-mediating protein family.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation occurs essentially on serine residues. Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-372 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-372 disrupts the URI1-PPP1CC complex in the mitochondrion, releaves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity and preventing sustained S6K1-dependent signaling.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Cell projection > dendrite. Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 at mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • C19orf2 antibody
    • Chromosome 19 open reading frame 2 antibody
    • NNX3 antibody
    • NNX3 protein antibody
    • PPP1R19 antibody
    • Protein NNX3 antibody
    • Protein phosphatase 1 regulatory subunit 19 antibody
    • RMP antibody
    • RMP_HUMAN antibody
    • RNA polymerase II subunit 5 mediating protein antibody
    • RNA polymerase II subunit 5-mediating protein antibody
    • RPB5 mediating protein antibody
    • RPB5-mediating protein antibody
    • Unconventional prefoldin RPB5 interactor 1 antibody
    • Unconventional prefoldin RPB5 interactor antibody
    • URI antibody
    • URI1 antibody
    • URI1, prefoldin-like chaperone antibody
    see all

Images

  • Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling C19orf2 with purified ab183310 at 1/80 dilution (1.09 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunohistochemical analysis of Human ovarian adenocarcinoma tissue labeling C19orf2 using ab183310 at a 1/100 dilution.

  • Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labeling C19orf2 with purified ab183310 at 1/80 dilution (1.09 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Flow cytometric analysis of Human 293 cells labeling C19orf2 using ab183310 at 1/100 (green), compared to a negative control rabbit IgG (blue).

References

ab183310 has not yet been referenced specifically in any publications.

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