Recombinant Anti-C19orf63 antibody [EPR13223] (ab181209)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13223] to C19orf63
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C19orf63 antibody [EPR13223]
See all C19orf63 primary antibodies -
Description
Rabbit monoclonal [EPR13223] to C19orf63 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A549, 293 and U87-MG cell lysates. Flow Cyt (intra): A549 cells. ICC/IF: Ramos cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13223 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/230.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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ICC/IF |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/230. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
ICC/IF
1/50 - 1/100. |
Target
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Tissue specificity
Present in serum (at protein level). Expressed in the pituitary gland; very low levels in other brain regions. -
Sequence similarities
Belongs to the EMC10 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 284361 Human
- Omim: 614545 Human
- SwissProt: Q5UCC4 Human
- Unigene: 448941 Human
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Alternative names
- Chromosome 19 open reading frame 63 antibody
- emc10 antibody
- EMC10_HUMAN antibody
see all
Images
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All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EMC10 knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaLanes 1-4: Merged signal (red and green). Green - ab181209 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.
ab181209 Anti-C19orf63 antibody [EPR13223] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab181209 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Intracellular flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling C19orf63 with ab181209 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Immunofluorescent analysis of Ramos cells (-20°C Acetone-fixed) labeling C19orf63 with ab181209 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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All lanes : Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution
Lane 1 : 293 cell lysate
Lane 2 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa -
Anti-C19orf63 antibody [EPR13223] (ab181209) at 1/20000 dilution + A549 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181209 has been referenced in 2 publications.
- Coelho JPL et al. A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10:672 (2019). PubMed: 30737405
- Tang X et al. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest 127:4314-4325 (2017). PubMed: 29083321