Anti-C19orf66 antibody (ab122765)
Key features and details
- Rabbit polyclonal to C19orf66
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C19orf66 antibody -
Description
Rabbit polyclonal to C19orf66 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: KFHGKASSKK AGALMRKFGS DHTGVGRSIV YGVKQKDGQE LSNDLDAQDP PEDMKQDRDI QAVATSLLPL TEANLRMFQR AQDD, corresponding to amino acids 18-101 of Human C19orf66.
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Positive control
- IHC-P: Human liver, prostate, Fallopian tube and testis tissue; ICC/IF: U-2 OS whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Sequence similarities
Belongs to the UPF0515 family. - Information by UniProt
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Database links
- Entrez Gene: 55337 Human
- SwissProt: Q9NUL5 Human
- Unigene: 655613 Human
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Alternative names
- C19orf66 antibody
- Chromosome 19 open reading frame 66 antibody
- CS066_HUMAN antibody
- UPF0515 protein C19orf66 antibody
Images
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Immunohistochemical analysis of human liver tissue labeling C19orf66 in the cytoplasm of hepatocytes with ab122765 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling C19orf66 in the nucleoplasm with ab122765 at 2 µg/ml.
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Immunohistochemical analysis of human testis tissue labeling C19orf66 in the cytoplasm of Leydig cells and of cells in seminiferous ducts with ab122765 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human Fallopian tube tissue labeling C19orf66 in the cytoplasm of glandular cells with ab122765 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human prostate tissue labeling C19orf66 in the cytoplasm of glandular cells with ab122765 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122765 has been referenced in 1 publication.
- Lee S et al. The SARS-CoV-2 RNA interactome. Mol Cell 81:2838-2850.e6 (2021). PubMed: 33989516