Key features and details
- Rabbit polyclonal to C1D
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-C1D antibody
See all C1D primary antibodies
DescriptionRabbit polyclonal to C1D
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Orangutan
- WB: Jurkat, Raji and NCI-H929 whole cell lysates. ICC/IF: U-2 OS cells. IHC-P: Mouse prostate and intestine tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.03% Proclin 300
Constituents: 79.97% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab227860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 16 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionPlays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. Can activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. Can induce apoptosis in a p53/TP53 dependent manner. May regulate the TRAX/TSN complex formation. Potentiates transcriptional repression by NR1D1 and THRB.
Tissue specificityUbiquitous. Expressed at very high levels in the hippocampus, medulla oblongata, mammary gland, thyroid and salivary gland. Expressed at high levels in the fetal; lung, liver and kidney. Expressed at low levels in skeletal muscle, appendix, heart, lung and colon.
Sequence similaritiesBelongs to the C1D family.
modificationsPhosphorylated by PRKDC.
Cellular localizationCytoplasm. Nucleus > nucleolus. EXOSC10 is required for nucleolar localization.
- Information by UniProt
- 1110036E10Rik antibody
- AI875855 antibody
- C1D antibody
Paraffin-embedded mouse prostate tissue stained for C1D using ab227860 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-C1D antibody (ab227860) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa
15 % SDS-PAGE gel.
U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for C1D (green) using ab227860 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
Paraffin-embedded mouse intestine tissue stained for C1D using ab227860 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227860 has not yet been referenced specifically in any publications.