Key features and details
- Rabbit polyclonal to C1GALT1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-C1GALT1 antibody
See all C1GALT1 primary antibodies
DescriptionRabbit polyclonal to C1GALT1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human C1GALT1 aa 115-144 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9NS00
- IHC-P: Human kidney tissue. WB: U-251 MG cell lysate; Mouse kidney lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 42 kDa.|
RelevanceC1GALT1 (Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1) is a glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R. Core 1 is a precursor for many extended O-glycans in glycoproteins. C1GALT1 plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
Cellular localizationMembrane; Single-pass type II membrane protein.
- B3Gal T8 antibody
- Beta 1 3 galactosyltransferase antibody
- C1GALT antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for C1GALT1 using ab230837 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-C1GALT1 antibody (ab230837) at 1/1000 dilution
Lane 1 : U-251 MG (human brain glioma cell line) cell lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230837 has not yet been referenced specifically in any publications.