Product nameAnti-C1orf135 antibody
DescriptionRabbit polyclonal to C1orf135
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- HEK293T transfected lysate. Human bone marrow tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- C1orf135 antibody
- CA135_HUMAN antibody
- Uncharacterized protein C1orf135 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab122014 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-C1orf135 antibody (ab122014) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : C1orf135 (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate
Developed using the ECL technique.
ab122014, at a 1/50 dilution, staining C1orf135 in paraffin-embedded Human bone marrow, by Immunohistochemistry.
ab122014 has not yet been referenced specifically in any publications.