Key features and details
- Rabbit polyclonal to C1orf177
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C1orf177 antibody
See all C1orf177 primary antibodies
DescriptionRabbit polyclonal to C1orf177
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: HEK-293T transfected with C1orf177 with C-terminal myc-DDK tag, cell lysate. IHC-P: Human testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab252956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceThe specific function of C1orf177 is not yet known.
- Chromosome 1 open reading frame 177 antibody
- FLJ40201 antibody
- Hypothetical protein LOC163747 antibody
- Uncharacterized protein C1orf177 antibody
Paraffin-embedded human testis tissue stained for C1orf177 using ab252956 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-C1orf177 antibody (ab252956) at 0.4 µg/ml
Lane 1 : Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T transfected with C1orf177 with C-terminal myc-DDK tag, cell lysate
Predicted band size: 48 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252956 has not yet been referenced specifically in any publications.