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Rabbit monoclonal [EPR14325] to C1orf57
Recombinant fragment within Human C1orf57 aa 1-150. The exact sequence is proprietary.
Database link: Q9BSD7
U87-MG and Jurkat cell lysates; Jurkat cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
C1orf57 (Chromosome 1 open reading frame 57) belongs to the UPF0334 family. The function of C1orf57 is unknown.
Chromosome 1 open reading frame 57 antibody
HCR NTPase antibody
Western blot - Anti-C1orf57 antibody [EPR14325] (ab182154)
All lanes : Anti-C1orf57 antibody [EPR14325] (ab182154) at 1/2000 dilution Lane 1 : U87-MG cell lysate Lane 2 : Jurkat cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution Predicted band size: 21 kDa
Flow Cytometry - Anti-C1orf57 antibody [EPR14325] (ab182154)
Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling C1orf57 with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunoprecipitation - Anti-C1orf57 antibody [EPR14325] (ab182154)
Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"