Recombinant
RabMAb

Recombinant Anti-C1orf69/IBA57 antibody [EPR11758(2)] (ab180161)

Overview

  • Product name

    Anti-C1orf69/IBA57 antibody [EPR11758(2)]
    See all C1orf69/IBA57 primary antibodies
  • Description

    Rabbit monoclonal [EPR11758(2)] to C1orf69/IBA57
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C1orf69/IBA57 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q5T440

  • Positive control

    • Raji, A431, Caco 2, 293T, HepG2 and Human fetal heart and liver lysates; Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as C1orf69

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11758(2)
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab180161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Required for normal heme biosynthesis.
    • Sequence similarities

      Belongs to the gcvT family. CAF17 subfamily.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • C1orf69 antibody
      • CAF17_HUMAN antibody
      • Chromosome 1 open reading frame 69 antibody
      • FLJ12734 antibody
      • FLJ13849 antibody
      • IBA57 antibody
      • IBA57 homolog iron sulfur cluster assembly antibody
      • IBA57 iron sulfur cluster assembly homolog antibody
      • IBA57 iron sulfur cluster assembly homolog (S. cerevisiae) antibody
      • IBA57, S. cerevisiae, homolog of antibody
      • Iron sulfur cluster assembly factor for biotin synthase and aconitase like antibody
      • iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins, with a mass of 57kDa antibody
      • Iron-sulfur cluster assembly factor homolog antibody
      • Mitochondrial proteins with a mass of 57kDa antibody
      • MMDS3 antibody
      • Putative transferase C1orf69 mitochondrial antibody
      • Putative transferase CAF17, mitochondrial antibody
      • SPG74 antibody
      see all

    Images

    • All lanes : Anti-C1orf69/IBA57 antibody [EPR11758(2)] (ab180161) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Raji cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Caco 2 cell lysate
      Lane 5 : 293T cell lysate
      Lane 6 : HepG2 cell lysate
      Lane 7 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 38 kDa

    • Flow cytometric analysis of permeabilized Raji cells labelling C1orf69/IBA57 using ab180161 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab180161 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180161.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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