• Product name
  • Description
    Rabbit polyclonal to C1orf77
  • Host species
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 of Human C1orf77 using the numbering given in entry NP_056422.2.

  • Positive control
    • Whole cell lysate from Jurkat, 293T and HeLa cells.



Our Abpromise guarantee covers the use of ab123603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 26 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Plays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
      Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity (PubMed:23299939).
    • Tissue specificity
      Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells (PubMed:25284789).
    • Post-translational
      Asymmetrically methylated by PRMT1 (PubMed:25284789). Symmetrically methylated by PRMT5.
    • Cellular localization
      Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle. Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).
    • Information by UniProt
    • Database links
    • Alternative names
      • C1orf77 antibody
      • chromatin target of chromatin target of PRMT1 protein antibody
      • Chromatin target of PRMT1 antibody
      • Chromatin target of PRMT1 protein antibody
      • Chromosome 1 open reading frame 77 antibody
      • CHTOP antibody
      • CHTOP_HUMAN antibody
      • DKFZp547E1010 antibody
      • FL- RP1-178F15.2 antibody
      • FL-SRAG antibody
      • FOP antibody
      • Friend of PRMT1 antibody
      • Friend of PRMT1 protein antibody
      • HT031 antibody
      • MGC131924 antibody
      • MGC86949 antibody
      • OTTHUMP00000035122 antibody
      • OTTHUMP00000035123 antibody
      • Pp7704 antibody
      • RP1 178F15.2 antibody
      • Small arginine- and glycine-rich protein antibody
      • Small protein rich in arginine and glycine antibody
      • SRAG antibody
      • SRAG-3 antibody
      • SRAG-5 antibody
      see all


    • All lanes : Anti-C1orf77 antibody (ab123603) at 0.4 µg/ml

      Lane 1 : Whole cell lysate from Jurkat cells at 50 µg
      Lane 2 : Whole cell lysate from Jurkat cells at 15 µg
      Lane 3 : Whole cell lysate from 293T cells at 50 µg
      Lane 4 : Whole cell lysate from HeLa cells at 50 µg

      Developed using the ECL technique.

      Predicted band size: 26 kDa

      Exposure time: 30 seconds


    ab123603 has not yet been referenced specifically in any publications.

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