Product nameAnti-C1orf77/FOP antibody
See all C1orf77/FOP primary antibodies
DescriptionRabbit polyclonal to C1orf77/FOP
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
- Jurkat, 293T and HeLa whole cell lysates.
This product was previously labelled as C1orf77, Fop
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176719 was affinity purified using an epitope specific to C1orf77/FOP immobilized on solid support.
Our Abpromise guarantee covers the use of ab176719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 26 kDa.|
FunctionPlays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity (PubMed:23299939).
Tissue specificityExpressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells (PubMed:25284789).
modificationsAsymmetrically methylated by PRMT1 (PubMed:25284789). Symmetrically methylated by PRMT5.
Cellular localizationNucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle. Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).
- Information by UniProt
- C1orf77 antibody
- chromatin target of chromatin target of PRMT1 protein antibody
- Chromatin target of PRMT1 antibody
All lanes : Anti-C1orf77/FOP antibody (ab176719) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutes
ab176719 has not yet been referenced specifically in any publications.