Key features and details
- Rabbit polyclonal to C1orf77/FOP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-C1orf77/FOP antibody
See all C1orf77/FOP primary antibodies
DescriptionRabbit polyclonal to C1orf77/FOP
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human C1orf77/FOP aa 179-248.
Database link: Q9Y3Y2
- WB: Mouse heart and kidney tissues. IHC-P: Human endometrial cancer and glioma cancer tissues.
This product was previously labelled as C1orf77, Fop
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 26, 52 kDa (predicted molecular weight: 26 kDa).|
FunctionPlays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity (PubMed:23299939).
Tissue specificityExpressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells (PubMed:25284789).
modificationsAsymmetrically methylated by PRMT1 (PubMed:25284789). Symmetrically methylated by PRMT5.
Cellular localizationNucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle. Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).
- Information by UniProt
- C1orf77 antibody
- chromatin target of chromatin target of PRMT1 protein antibody
- Chromatin target of PRMT1 antibody
All lanes : Anti-C1orf77/FOP antibody (ab222861) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26 kDa
Paraffin-embedded human endometrial cancer tissue stained for C1orf77/FOP using ab222861 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human glioma cancer tissue stained for C1orf77/FOP using ab222861 at 1/100 dilution in immunohistochemical analysis.
ab222861 has not yet been referenced specifically in any publications.