• Product name

  • Description

    Rabbit polyclonal to C1orf77/FOP
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human C1orf77/FOP aa 179-248.
    Database link: Q9Y3Y2

  • Positive control

    • WB: Mouse heart and kidney tissues. IHC-P: Human endometrial cancer and glioma cancer tissues.
  • General notes

     This product was previously labelled as C1orf77, Fop




Our Abpromise guarantee covers the use of ab222861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 26, 52 kDa (predicted molecular weight: 26 kDa).


  • Function

    Plays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
    Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity (PubMed:23299939).
  • Tissue specificity

    Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells (PubMed:25284789).
  • Post-translational

    Asymmetrically methylated by PRMT1 (PubMed:25284789). Symmetrically methylated by PRMT5.
  • Cellular localization

    Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle. Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332).
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf77 antibody
    • chromatin target of chromatin target of PRMT1 protein antibody
    • Chromatin target of PRMT1 antibody
    • Chromatin target of PRMT1 protein antibody
    • Chromosome 1 open reading frame 77 antibody
    • CHTOP antibody
    • CHTOP_HUMAN antibody
    • DKFZp547E1010 antibody
    • FL- RP1-178F15.2 antibody
    • FL-SRAG antibody
    • FOP antibody
    • Friend of PRMT1 antibody
    • Friend of PRMT1 protein antibody
    • HT031 antibody
    • MGC131924 antibody
    • MGC86949 antibody
    • OTTHUMP00000035122 antibody
    • OTTHUMP00000035123 antibody
    • Pp7704 antibody
    • RP1 178F15.2 antibody
    • Small arginine- and glycine-rich protein antibody
    • Small protein rich in arginine and glycine antibody
    • SRAG antibody
    • SRAG-3 antibody
    • SRAG-5 antibody
    see all


  • All lanes : Anti-C1orf77/FOP antibody (ab222861) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 26 kDa

  • Paraffin-embedded human endometrial cancer tissue stained for C1orf77/FOP using ab222861 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human glioma cancer tissue stained for C1orf77/FOP using ab222861 at 1/100 dilution in immunohistochemical analysis.


ab222861 has not yet been referenced specifically in any publications.

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