Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [4.8] to C1q
- Suitable for: IHC-FoFr
- Knockout validated
- Reacts with: Mouse
Product nameAnti-C1q antibody [4.8]
See all C1q primary antibodies
DescriptionRabbit monoclonal [4.8] to C1q
Tested applicationsSuitable for: IHC-FoFrmore details
Species reactivityReacts with: Mouse
Full length native protein (purified) corresponding to Mouse C1q.
- IHC: Mouse brain tissue (adult) .
This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University:
Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use a concentration of 1 µg/ml.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Involvement in diseaseDefects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
- Information by UniProt
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
ab182451 (1/500) staining C1q in 15µm coronal brain sections from postnatal day 90 C1qA KO mice and WT litter-mates (Botto et al., 1998) by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton. An Alexa Flour-conjugated Goat anti-Mouse IgG polyclonal (1/500) was used as the secondary antibody. Positive staining on wild type (WT) mouse cerebral cortex, and no staining on knockout (KO) mouse cerebral cortex.
ab182451 has been referenced in 21 publications.
- Haure-Mirande JV et al. Integrative approach to sporadic Alzheimer's disease: deficiency of TYROBP in cerebral Aß amyloidosis mouse normalizes clinical phenotype and complement subnetwork molecular pathology without reducing Aß burden. Mol Psychiatry 24:431-446 (2019). PubMed: 30283032
- Jay TR et al. TREM2 is required for microglial instruction of astrocytic synaptic engulfment in neurodevelopment. Glia 67:1873-1892 (2019). PubMed: 31265185
- Bieri G et al. LRRK2 modifies a-syn pathology and spread in mouse models and human neurons. Acta Neuropathol 137:961-980 (2019). PubMed: 30927072
- Adaikkan C et al. Gamma Entrainment Binds Higher-Order Brain Regions and Offers Neuroprotection. Neuron 102:929-943.e8 (2019). PubMed: 31076275
- Vukojicic A et al. The Classical Complement Pathway Mediates Microglia-Dependent Remodeling of Spinal Motor Circuits during Development and in SMA. Cell Rep 29:3087-3100.e7 (2019). PubMed: 31801075