Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Post mortem brain tissue)
Permeabilization
Yes - EtOH/H2O2
Specification
Post mortem brain tissue
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 10µg/mL · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Diluent: TBS/50% blocking buffer
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
No detectable C1q labelling in human post-mortem brain tissue. Microglia within the section were labelled with Iba-1 as a positive control for the protocol.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab182451 and it seems that this antibody does not detect C1q under these experimental conditions. Thank you for your time.
We would welcome feedback from other customers.
Abcam user community
Verified customer
Submitted Aug 15 2016