Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9
Specification
Brain
Blocking step
Casein as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 94°C
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Biocare Mach 2 Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
No staining within the mouse brain with IHC-P method
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab182451. As the product had not yet been tested for IHC-P we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Inotiv Boulder
Verified customer
Submitted Aug 26 2019