Key features and details
- Rabbit monoclonal [4.8] to C1q - Low endotoxin, Azide free
- Suitable for: IHC-FoFr
- Knockout validated
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-C1q antibody [4.8] - Low endotoxin, Azide free
See all C1q primary antibodies
DescriptionRabbit monoclonal [4.8] to C1q - Low endotoxin, Azide free
Tested applicationsSuitable for: IHC-FoFrmore details
Species reactivityReacts with: Mouse
Full length native protein (purified).
- IHC: Mouse brain tissue (adult) .
ab227072 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University: Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab227072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Involvement in diseaseDefects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
- Information by UniProt
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227072 has been referenced in 5 publications.
- Silverman SM et al. C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury. Mol Neurodegener 11:24 (2016). IHC-Fr ; Mouse . PubMed: 27008854
- Hong S et al. Complement and microglia mediate early synapse loss in Alzheimer mouse models. Science 352:712-6 (2016). IHC ; Mouse . PubMed: 27033548
- Lui H et al. Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation. Cell 165:921-35 (2016). ICC/IF ; Mouse . PubMed: 27114033
- Chung WS et al. Novel allele-dependent role for APOE in controlling the rate of synapse pruning by astrocytes. Proc Natl Acad Sci U S A 113:10186-91 (2016). IHC-P ; Mouse . PubMed: 27559087
- Stephan AH et al. A dramatic increase of C1q protein in the CNS during normal aging. J Neurosci 33:13460-74 (2013). IHC-FoFr ; Mouse . PubMed: 23946404