Overview

  • Product name

    Anti-C1q antibody [4.8] - Low endotoxin, Azide free
    See all C1q primary antibodies
  • Description

    Rabbit monoclonal [4.8] to C1q - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length native protein (purified).

  • Positive control

    • IHC: Mouse brain tissue (adult) .
  • General notes

    ab227072 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University: Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.

Properties

Applications

Our Abpromise guarantee covers the use of ab227072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease

    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Images

  • This IHC data was generated using the same anti-C1q antibody clone, 4.8, in a different buffer formulation (cat# ab182451).

    Immunohistochemical analysis of perfusion-fixed mouse brain tissue labeling C1q with ab182451 at 1 μg/ml. DAPI staining (blue).

References

This product has been referenced in:

  • Silverman SM  et al. C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury. Mol Neurodegener 11:24 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27008854) »
  • Hong S  et al. Complement and microglia mediate early synapse loss in Alzheimer mouse models. Science 352:712-6 (2016). IHC ; Mouse . Read more (PubMed: 27033548) »
See all 5 Publications for this product

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