Overview

  • Product name
    Anti-C1q antibody [7H8]
    See all C1q primary antibodies
  • Description
    Rat monoclonal [7H8] to C1q
  • Host species
    Rat
  • Specificity
    Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
  • Tested applications
    Suitable for: ICC, IHC-FoFr, ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This information is considered to be commercially sensitive

  • Positive control
    • C1q can be found on macrophages, follicular dendritic cells, interdigitating cells and further cells of the monocyte-macrophage lineage. These types of cells can be used as positive control.

Properties

Applications

Our Abpromise guarantee covers the use of ab11861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10.

Target

  • Function
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease
    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Images

  • ab11861 staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 1% paraformaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS) for 2 hours at 24°C. An undiluted Alexa Fluor®488-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.

    See Abreview

References

This product has been referenced in:
  • Takahashi H  et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol 133:785-807 (2017). Read more (PubMed: 28070672) »
  • Bandini S  et al. The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis. Oncoimmunology 5:e1253653 (2016). Read more (PubMed: 28123895) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Haoran Zha

Verified customer

Submitted Mar 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (Neuro2a cell line)
Specification
Neuro2a cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 28 2012

Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 26 2012

Answer

I checked ab71940 and you are correct, it is a similar situation. Unless there is a specific note on the product datasheet stating it has low endotoxin levels, then antibodies are not prepared with endotoxin levels in mind and likely have high, albeit unknown, levels. I hope this is helpful. Please contact me again if you have any further questions.

Read More

Answer

Thank you for your enquiry. ab11861 has not been tested for endotoxin levels. It is not produced specifically as a low endotoxin product. Here is a link to the only reference we know of using this antibody in functional assays: http://www.jleukbio.org/content/85/1/55.full?sid=93020101-044d-4c93-a7ba-70f8391561f9 We are not aware of other studies using clone [7H8] to neutralize C1q. I hope this is helpful. Please contact me again if you have any further questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 17 2011

Answer

Thank you for your enquiry regarding ab11861. The antibody is supplied at a concentration of 0.1mg/ml and we recommend a dilution of 1/10 but we predict it will work at higher dilutions, so I would recommend to try 1/10, 1/50, 1/100, 1/200 and 1/500 and optimise following the results of that preliminary experiment. I hope this information will help you, please do not hesitate to contact me again if I can be of further assistance,

Read More

Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. According to the information that we have available this antibody demonstrates reactivity to murine plasma but is yet to show reactivity towards human plasma. I have read your technical questionaire and I have a few comments. You are performing an approach that I would largely recommend. However, I would appreciate it if you could tell me whether you consider the mass of protein loaded equal between the human and mouse serum. Can you tell me whether you performed a ponceau staining and whether this suggested equivocal loading. I am currently in touch with the source of this antibody to verify the documented the reactivity that we have on the datasheet.

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Question
Answer

Thank you for your enquiry regarding ab11861. The exact epitope recognized by the antibody is not known. There are no studies performed to find more information about the epitope recognized by ab11861, therefore, we are not able to give you more information about the recognition of the B chain of C1q. We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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