- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-C1q antibody [7H8]
See all C1q primary antibodies -
DescriptionRat monoclonal [7H8] to C1q
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Host speciesRat
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SpecificityImmunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
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Tested applicationsSuitable for: ICC, IHC-FoFr, ICC/IF, ELISA, IHC-Frmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
This information is considered to be commercially sensitive
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Positive control
- C1q can be found on macrophages, follicular dendritic cells, interdigitating cells and further cells of the monocyte-macrophage lineage. These types of cells can be used as positive control.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notes0.2 µm filtered
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ClonalityMonoclonal
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Clone number7H8
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab11861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/10. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | 1/10. |
Target
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FunctionC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
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Involvement in diseaseDefects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
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Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Entrez Gene: 12259 Mouse
- Entrez Gene: 12260 Mouse
- Entrez Gene: 12262 Mouse
- Omim: 120550 Human
- Omim: 120570 Human
see all -
Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)](https://www.abcam.com/ps/products/11/ab11861/Images/ab11861-222846-ab11861iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)This image is courtesy of an anonymous Abreviewab11861 staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 1% paraformaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS) for 2 hours at 24°C. An undiluted Alexa Fluor®488-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.
References
This product has been referenced in:
- Zhang Z et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . Read more (PubMed: 24191055) »
- Linnartz B et al. Sialic acid on the neuronal glycocalyx prevents complement C1 binding and complement receptor-3-mediated removal by microglia. J Neurosci 32:946-52 (2012). ICC/IF ; Mouse . Read more (PubMed: 22262892) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"