For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    c1q-antibody-7h8-ab11861.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Complement Classical Pathway
Share by email

Anti-C1q antibody [7H8] (ab11861)

  • Datasheet
  • SDS
Reviews (5)Q&A (8)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)

    Key features and details

    • Rat monoclonal [7H8] to C1q
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG1

    You may also be interested in

    Primary
    Product image
    Anti-C3 antibody [11H9] (ab11862)
    Primary
    Product image
    Anti-C1q antibody [4.8] (ab182451)
    Pair
    Product image
    Mouse Albumin Matched Antibody Pair Kit (ab210890)

    View more associated products

    Overview

    • Product name

      Anti-C1q antibody [7H8]
      See all C1q primary antibodies
    • Description

      Rat monoclonal [7H8] to C1q
    • Host species

      Rat
    • Specificity

      Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
    • Tested Applications & Species

      Application Species
      IHC-Fr
      Mouse
      See all applications and species data
    • Immunogen

      This information is considered to be commercially sensitive.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      0.2 µm filtered
    • Clonality

      Monoclonal
    • Clone number

      7H8
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Signal Transduction
      • Antibodies
      • c1q

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11861 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-Fr
    Mouse
    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.

    Target

    • Function

      C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
    • Involvement in disease

      Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • Post-translational
      modifications

      O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02745 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12259 Mouse
      • Entrez Gene: 12260 Mouse
      • Entrez Gene: 12262 Mouse
      • SwissProt: P14106 Mouse
      • SwissProt: P98086 Mouse
      • SwissProt: Q02105 Mouse
      • Unigene: 439957 Mouse
      • Alternative names

        • C1qa antibody
        • C1QA_HUMAN antibody
        • C1QB antibody
        • C1QC antibody
        • Complement C1q subcomponent subunit A antibody
        • Complement C1q subcomponent subunit B antibody
        • Complement C1q subcomponent subunit C antibody
        • Complement component 1 q subcomponent alpha polypeptide antibody
        • Complement component C1q A chain antibody
        • Complement component C1q B chain antibody
        • Complement component C1q C chain antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)
        Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)
        Immunohistochemistry analysis of 4% PFA fixed and sucrose infiltrated frozen mouse spleen endothelial blood vessels with ab11861 at 1/500.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (10)

      Publishing research using ab11861? Please let us know so that we can cite the reference in this datasheet.

      ab11861 has been referenced in 10 publications.

      • Takahashi H  et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol 133:785-807 (2017). PubMed: 28070672
      • Bandini S  et al. The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis. Oncoimmunology 5:e1253653 (2016). PubMed: 28123895
      • Zhang Z  et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . PubMed: 24191055
      • Linnartz B  et al. Sialic acid on the neuronal glycocalyx prevents complement C1 binding and complement receptor-3-mediated removal by microglia. J Neurosci 32:946-52 (2012). ICC/IF ; Mouse . PubMed: 22262892
      • Lopez ME  et al. Neuronal and epithelial cell rescue resolves chronic systemic inflammation in the lipid storage disorder Niemann-Pick C. Hum Mol Genet : (2012). ICC/IF ; Mouse . PubMed: 22493001
      • Ankeny DP  et al. B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice. J Clin Invest 119:2990-9 (2009). IHC-FoFr ; Mouse . PubMed: 19770513
      • O' Reilly LA  et al. Membrane-bound Fas ligand only is essential for Fas-induced apoptosis. Nature 461:659-63 (2009). IHC-Fr ; Mouse . PubMed: 19794494
      • Lobsiger CS  et al. Toxicity from different SOD1 mutants dysregulates the complement system and the neuronal regenerative response in ALS motor neurons. Proc Natl Acad Sci U S A 104:7319-26 (2007). IHC-FoFr ; Mouse . PubMed: 17463094
      • Suresh MV  et al. Role of the property of C-reactive protein to activate the classical pathway of complement in protecting mice from pneumococcal infection. J Immunol 176:4369-74 (2006). ELISA ; Mouse . PubMed: 16547275
      • Wasiliu M  et al. Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 8:615-22 (1989). PubMed: 2482249

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 13 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C1q antibody [7H8]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
      Permeabilization
      No
      Specification
      kidney
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Haoran Zha

      Verified customer

      Submitted Mar 14 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-C1q [7H8] antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
      Sample
      Human Cell (SHSY5Y)
      Specification
      SHSY5Y
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 28 2014

      Immunocytochemistry abreview for Anti-C1q antibody [7H8]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Mouse Cell (Neuro2a cell line)
      Specification
      Neuro2a cell line
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 28 2012

      Immunocytochemistry abreview for Anti-C1q antibody [7H8]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cell (Kelly Neuroblastoma cell line)
      Specification
      Kelly Neuroblastoma cell line
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 26 2012

      Question

      Thank you for the quick response. I also sent another email almost immediately after this one yesterday about another one of your antibodies (Anti-C1q antibody [JL-1] (ab71940)). I had asked about endotoxin testing so am I to assume the same for this antibody, that testing hasn’t been done nor is it specifically produced as a low endotoxin product?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 21 2011

      Answer

      I checked ab71940 and you are correct, it is a similar situation. Unless there is a specific note on the product datasheet stating it has low endotoxin levels, then antibodies are not prepared with endotoxin levels in mind and likely have high, albeit unknown, levels. I hope this is helpful. Please contact me again if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Dec 21 2011

      Question

      Hello, I have a few questions regarding Anti-C1q antibody [7H8]: does this antibody neutralize C1q and has endotoxin testing been done on this product? I am interested in this antibody for in vivo studies and need to find a low endotoxin batch.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 21 2011

      Answer

      Thank you for your enquiry. ab11861 has not been tested for endotoxin levels. It is not produced specifically as a low endotoxin product. Here is a link to the only reference we know of using this antibody in functional assays: http://www.jleukbio.org/content/85/1/55.full?sid=93020101-044d-4c93-a7ba-70f8391561f9 We are not aware of other studies using clone [7H8] to neutralize C1q. I hope this is helpful. Please contact me again if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Dec 21 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-C1q antibody [7H8]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Spleen)
      Specification
      Spleen
      Fixative
      Paraformaldehyde
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 17 2011

      Question

      I'm doing IHC staining on monocytogenes-listeria-infected mouse brain cryosections. I know that generally I've got to find my own dilutions for your antibody, but do you have a recommended starting range? In the absence of hearing anything from you, I'm going to start out at 1:200, 1:400, 1:800. I appreciate any more specific knowledge you might have. Thanks

      Read More

      Abcam community

      Verified customer

      Asked on May 31 2006

      Answer

      Thank you for your enquiry regarding ab11861. The antibody is supplied at a concentration of 0.1mg/ml and we recommend a dilution of 1/10 but we predict it will work at higher dilutions, so I would recommend to try 1/10, 1/50, 1/100, 1/200 and 1/500 and optimise following the results of that preliminary experiment. I hope this information will help you, please do not hesitate to contact me again if I can be of further assistance,

      Read More

      Abcam Scientific Support

      Answered on Jun 01 2006

      Question

      BATCH NUMBER 145426 ORDER NUMBER 142660 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE mouse and human plasma PRIMARY ANTIBODY ab 11861 same buffer overnight at 4 C at 1/10 DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED The human plasma is positive with a band around 25kda, no signal in mouse plasma and in my samples. Same samples give a good signal for other proteins of the complement like C1r ANTIBODY STORAGE CONDITIONS 4 SAMPLE PREPARATION plasma including protease inhibitor AMOUNT OF PROTEIN LOADED 2 ?l of plasma ELECTROPHORESIS/GEL CONDITIONS reduging 4-20% SDS-PAGE TRANSFER AND BLOCKING CONDITIONS PVDF TBS tween 0.1%, 5% dry milk 1h SECONDARY ANTIBODY anti rat from jackson lab 1h in the same buffer HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? tried 3% BSA for blocking

      Read More

      Abcam community

      Verified customer

      Asked on May 10 2006

      Answer

      Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. According to the information that we have available this antibody demonstrates reactivity to murine plasma but is yet to show reactivity towards human plasma. I have read your technical questionaire and I have a few comments. You are performing an approach that I would largely recommend. However, I would appreciate it if you could tell me whether you consider the mass of protein loaded equal between the human and mouse serum. Can you tell me whether you performed a ponceau staining and whether this suggested equivocal loading. I am currently in touch with the source of this antibody to verify the documented the reactivity that we have on the datasheet.

      Read More

      Abcam Scientific Support

      Answered on May 12 2006

      Question

      The customer would like to know if either of these antibodies recognizes C1q B.

      Read More

      Abcam community

      Verified customer

      Asked on May 09 2005

      Answer

      Thank you for your enquiry regarding ab11861. The exact epitope recognized by the antibody is not known. There are no studies performed to find more information about the epitope recognized by ab11861, therefore, we are not able to give you more information about the recognition of the B chain of C1q. We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on May 17 2005

      1-10 of 13 Abreviews or Q&A

      •  Previous
      • 1
      • 2
      • Next 

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.