Anti-C1q antibody [7H8] (ab11861)

Reviews (5)Q&A (8)References (10)

Overview

  • Product name

    Anti-C1q antibody [7H8]
    See all C1q primary antibodies

  • Description

    Rat monoclonal [7H8] to C1q

  • Host species

    Rat

  • Specificity

    Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.

  • Tested applications

    Suitable for: ICC, IHC-FoFr, ICC/IF, ELISA, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    This information is considered to be commercially sensitive

  • Positive control

    • IHC-P: mouse kidney; ICC/ IF: mouse spleen and human SHSY5Y cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab11861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10.

Target

  • Function

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

  • Involvement in disease

    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.

  • Post-translational
    modifications

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)
    Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)

    ab11861 staining C1q in mouse spleen cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/10 in PBS) for 16 hours at 4°C. A Donkey Anti-Rat AF488 (Green) was used as the secondary antibody. Counterstained with Hoescht 33342 (Blue).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1q antibody [7H8] (ab11861)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1q antibody [7H8] (ab11861)

    Paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue stained for C1q using ab11861 at 1/25 dilution in immunohistochemical analysis, followed by ab11668 at 1/300 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.0.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)
    Immunocytochemistry/ Immunofluorescence - Anti-C1q antibody [7H8] (ab11861)This image is courtesy of an anonymous Abreview

    ab11861 staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 1% paraformaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS) for 2 hours at 24°C. An undiluted Alexa Fluor®488-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.

    See Abreview

References

This product has been referenced in:

  • Takahashi H  et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol 133:785-807 (2017). Read more (PubMed: 28070672) »
  • Bandini S  et al. The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis. Oncoimmunology 5:e1253653 (2016). Read more (PubMed: 28123895) »
See all 10 Publications for this product

Publishing research using ab11861? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 13 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C1q antibody [7H8]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Haoran Zha

Verified customer

Submitted Mar 14 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-C1q [7H8] antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 28 2014

Immunocytochemistry abreview for Anti-C1q antibody [7H8]

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (Neuro2a cell line)
Specification
Neuro2a cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Sep 28 2012

Immunocytochemistry abreview for Anti-C1q antibody [7H8]

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Sep 26 2012

Question

Thank you for the quick response. I also sent another email almost immediately after this one yesterday about another one of your antibodies (Anti-C1q antibody [JL-1] (ab71940)). I had asked about endotoxin testing so am I to assume the same for this antibody, that testing hasn’t been done nor is it specifically produced as a low endotoxin product?

Read More
Answer

I checked ab71940 and you are correct, it is a similar situation. Unless there is a specific note on the product datasheet stating it has low endotoxin levels, then antibodies are not prepared with endotoxin levels in mind and likely have high, albeit unknown, levels. I hope this is helpful. Please contact me again if you have any further questions.

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Question

Hello, I have a few questions regarding Anti-C1q antibody [7H8]: does this antibody neutralize C1q and has endotoxin testing been done on this product? I am interested in this antibody for in vivo studies and need to find a low endotoxin batch.

Read More
Answer

Thank you for your enquiry. ab11861 has not been tested for endotoxin levels. It is not produced specifically as a low endotoxin product. Here is a link to the only reference we know of using this antibody in functional assays: http://www.jleukbio.org/content/85/1/55.full?sid=93020101-044d-4c93-a7ba-70f8391561f9 We are not aware of other studies using clone [7H8] to neutralize C1q. I hope this is helpful. Please contact me again if you have any further questions.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-C1q antibody [7H8]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Aug 17 2011

Question

I'm doing IHC staining on monocytogenes-listeria-infected mouse brain cryosections. I know that generally I've got to find my own dilutions for your antibody, but do you have a recommended starting range? In the absence of hearing anything from you, I'm going to start out at 1:200, 1:400, 1:800. I appreciate any more specific knowledge you might have. Thanks

Read More
Answer

Thank you for your enquiry regarding ab11861. The antibody is supplied at a concentration of 0.1mg/ml and we recommend a dilution of 1/10 but we predict it will work at higher dilutions, so I would recommend to try 1/10, 1/50, 1/100, 1/200 and 1/500 and optimise following the results of that preliminary experiment. I hope this information will help you, please do not hesitate to contact me again if I can be of further assistance,

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Question

BATCH NUMBER 145426 ORDER NUMBER 142660 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE mouse and human plasma PRIMARY ANTIBODY ab 11861 same buffer overnight at 4 C at 1/10 DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED The human plasma is positive with a band around 25kda, no signal in mouse plasma and in my samples. Same samples give a good signal for other proteins of the complement like C1r ANTIBODY STORAGE CONDITIONS 4 SAMPLE PREPARATION plasma including protease inhibitor AMOUNT OF PROTEIN LOADED 2 ?l of plasma ELECTROPHORESIS/GEL CONDITIONS reduging 4-20% SDS-PAGE TRANSFER AND BLOCKING CONDITIONS PVDF TBS tween 0.1%, 5% dry milk 1h SECONDARY ANTIBODY anti rat from jackson lab 1h in the same buffer HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? tried 3% BSA for blocking

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Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. According to the information that we have available this antibody demonstrates reactivity to murine plasma but is yet to show reactivity towards human plasma. I have read your technical questionaire and I have a few comments. You are performing an approach that I would largely recommend. However, I would appreciate it if you could tell me whether you consider the mass of protein loaded equal between the human and mouse serum. Can you tell me whether you performed a ponceau staining and whether this suggested equivocal loading. I am currently in touch with the source of this antibody to verify the documented the reactivity that we have on the datasheet.

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Question

The customer would like to know if either of these antibodies recognizes C1q B.

Read More
Answer

Thank you for your enquiry regarding ab11861. The exact epitope recognized by the antibody is not known. There are no studies performed to find more information about the epitope recognized by ab11861, therefore, we are not able to give you more information about the recognition of the B chain of C1q. We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

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1-10 of 13 Abreviews or Q&A

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