Recombinant Anti-C1q antibody [7H8] (ab11861)
![Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)](https://www.abcam.com/ps/products/11/ab11861/Images/ab11861-479940-anti-c1q-antibody-7h8-immunohistochemistry-frozen-sections.jpg "Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7H8] to C1q
- Suitable for: ICC/IF, ELISA, IHC-FoFr, ICC, IHC-Fr
- Reacts with: Mouse, Human
Overview
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Product name
Anti-C1q antibody [7H8]
See all C1q primary antibodies -
Description
Rat monoclonal [7H8] to C1q -
Host species
Rat -
Specificity
Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
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Tested applications
Suitable for: ICC/IF, ELISA, IHC-FoFr, ICC, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-Fr: P90 Wild-type mouse.
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General notes
This product was switched from a hybridoma to a recombinant production format on 25th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7H8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
| ICC | (2) |
Use at an assay dependent concentration.
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| IHC-Fr |
1/500.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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IHC-Fr
1/500. |
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Entrez Gene: 12259 Mouse
- Entrez Gene: 12260 Mouse
- Entrez Gene: 12262 Mouse
- Omim: 120550 Human
- Omim: 120570 Human
see all -
Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (ab11861)This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.
Positive staining on P90 Wild-type mouse, and no staining on P90 C1qa Knockout Mouse . Primary antibody was applied at a dilution of 1/500 and anti-VGLUT1 antibody. Secondary antibodies including goat anti rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 and goat anti guinea pig IgG (H+L) highly cross-adsorbed Alexa Fluor 488 were also employed at a dilution of 1/500.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab11861 has been referenced in 12 publications.
- Figueiredo CP et al. Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice. Nat Commun 10:3890 (2019). PubMed: 31488835
- Hata J et al. Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia. Sci Rep 9:20357 (2019). PubMed: 31889151
- Takahashi H et al. Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol 133:785-807 (2017). PubMed: 28070672
- Bandini S et al. The non-inflammatory role of C1q during Her2/neu-driven mammary carcinogenesis. Oncoimmunology 5:e1253653 (2016). PubMed: 28123895
- Zhang Z et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . PubMed: 24191055