Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7H8] to C1q - BSA and Azide free
- Suitable for: IHC-Fr
- Reacts with: Mouse
Product nameAnti-C1q antibody [7H8] - BSA and Azide free
See all C1q primary antibodies
DescriptionRat monoclonal [7H8] to C1q - BSA and Azide free
Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
Tested applicationsSuitable for: IHC-Frmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
- IHC-Fr: P90 Wild-type mouse.
ab255973 is the carrier-free version of ab11861.
This product was switched from a hybridoma to a recombinant production format on 25th October 2021.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.20
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Involvement in diseaseDefects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
- Information by UniProt
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
This data was developed using ab11861 the same antibody clone in a different buffer formulation.
Positive staining on P90 Wild-type mouse, and no staining on P90 C1qa Knockout Mouse . Primary antibody was applied at a dilution of 1/500 and anti-VGLUT1 antibody. Secondary antibodies including goat anti rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 and goat anti guinea pig IgG (H+L) highly cross-adsorbed Alexa Fluor 488 were also employed at a dilution of 1/500.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab255973 has not yet been referenced specifically in any publications.