Overview

  • Product name
    Anti-C1q antibody (FITC)
    See all C1q primary antibodies
  • Description
    Rabbit polyclonal to C1q (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with human C1q complement.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C1q isolated from the normal human serum.

  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab4223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21746929

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22393049

Target

  • Function
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease
    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

Images

  • C1q isolated from the NHS. This antibody reacts with the Human C1q complement. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.

  • ab4223, staining C1q in CHO-K1 cells transfected with M1-AQP4 or M23-AQP4 by Immunocytochemistry/ Immunofluorescence.

    Cells were incubated at room temperature with recombinant monoclonal NMO antibody and 120 µg/ml human C1q protein (ab96363). Cells were washed with PBS, fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with primary antibody against C1q (ab4223) at 1/200 dilution.

References

This product has been referenced in:
  • Burbano C  et al. Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus. Front Immunol 9:322 (2018). Read more (PubMed: 29545790) »
  • Fox S  et al. The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection. J Inflamm (Lond) 12:51 (2015). Read more (PubMed: 26357509) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Loading amount
0.06 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Serum (Serum)
Specification
Serum
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jul 15 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Dog Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jul 01 2014

Answer

Thank you for contacting us.

My colleague has confirmed that we do not currently OEM any of our products for resale. If you are interested in using our products to develop other assays such as kits, my colleagues at mailto:sales@abcam.com can help you with bulk discount information. Unfortunately, we would not be able to provide a quote for reselling the products directly.

The products fitting your description are:

ab4223: C1q antibody (FITC)
ab4212: C3c antibody (FITC)
ab79118: Human Kappa Chain antibody [SB81a] (FITC)
ab4208: Kappa light chain antibody (FITC)
ab9006: Lambda light chain antibody (FITC)
ab99809: Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)
ab99799: Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

The datasheets with the most current information and pricing are linked below. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

Gracias por contactarnos de nuevo, ¡no es ninguna molestia!

He revisado los artículos referenciados en la datasheet del producto, y para ICC/IF la dilución recomendada es de 1/200. Te copio el párrafo correspondiente al protocolo usado, por si pudiera resultar de utilidad a la clienta:

C1q and C9neo Immunofluorescence

CHO-K1 cells were plated onto coverslips in 24-well plates and grown at 37 °C/5% CO2 for 18–24 h. For C1q immunofluorescence, cells were washed with Hanks' BSS and incubated at room temperature for 30 min with 400 μl of Hanks' BSS containing 40 μg/ml rAb-58 and 120 μg/ml human C1q protein (Abcam, Cambridge, MA). Cells were washed with PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated Alexa Fluor 555 (http://www.jbc.org/cgi/redirect-inline?ad=Invitrogen) in 1% BSA. For C9neo immunofluorescence, cells were incubated with rAbs as above, together with 2% pooled normal human complement serum. After fixation and permeabilization, cells were incubated for 60 min with 0.1 μg/ml mouse monoclonal anti-C5b-9 (C9neo) antibody and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (both from Santa Cruz Biotechnology). Cells were then incubated for 30 min with 5 μg/ml donkey anti-mouse IgG-conjugated Alexa Fluor 488 and goat anti-rabbit IgG-conjugated Alexa Fluor 555 (http://www.jbc.org/cgi/redirect-inline?ad=Invitrogen) in 1% BSA.

Para Citometría no se menciona en el artículo la dilución empleada, pero nosotros siempre recomendamos usar, para anticuerpos purificados, como es este caso, una concentración de 1ug/ml.

El párrafo al respecto del artículo mencionado se adjunta a continuación:

C1q Deposition on Infected Erythrocytes.

Binding of complement component C1q IEs was measured as described previously (17). In brief, purified late-stage IEs were incubated in PBS (30 min at 4 °C) with or without either IgM or IgG (300 μg/mL), washed three times, incubated (30 min at 4 °C) in Veronal buffer (http://www.oxoid.com) supplemented with either purified human C1q (10 μg/mL; http://www.sigma-aldrich.com) or nonimmune plasma (10%) as the C1q source, washed three times, and labeled (30 min at 4 °C) with FITC-conjugated rabbit anti-human C1q (https://www.abcam.com), anti-human IgG, or anti-human IgM. After two washes in Veronal buffer, the labeled IEs were analyzed by flow cytometry as above.

Espero haber resuelto la duda de la clienta. En caso contrario, por favor no dudes en volverme a contactar, y estaré encantada de poder ayudar.

¡Que pases un día estupendo!

Read More

Question
Answer

Approximately 1 mg/ml

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up