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uses ab46191 as detection Ab in sELISA (untested) capture Ab is human monoclonal Ab (therapeutic Ab) - sees high signal when using no antigen (C1q), same as when using C1q. other capture Abs (also human, therapeutic) do not show signal in same setting explanation: above capture Ab might have show homology for Ab binding site has not been tested uses BSA for blocking Is immunogen composed of all 3 C1q subunits A, B and C?
Asked on Dec 22 2011
Thank you for contacting us. I received the following information from the supplying lab: The detailed information regarding the specifics of the immunogen (e.g. is the immunogen composed of all 3 C1q subunits A, B and C or just one of them?) are proprietary and we are not able to disclose this information. We can tell, however, that the immunogen is purified human C1 complement. It has been shown to have minimal cross reactivity with related serum proteins. We do know that this antibody reacts with beta-1-C and beta-1-A native antigen in fresh plasma. I am sorry to not have the exact information that you were looking for. Please do not hesitate to contact us if you need any more advice or information.
Answered on Dec 22 2011