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I would like to know what is the stability of the Anti-C1q antibody (HRP) (Catalog number ab46191), when aliquoted and stored at -20°C.
Asked on Feb 24 2012
Thank you for your enquiry.
I can confirm that this antibodyshouldnot to be frozen but stored at +4C.
We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased.
We can only guarantee our products to work for at least six month from the date of purchase when stored correctly.
Freezing (and thawing)this antibody might separate the HRP molecule from the antibody andwhich will lead to aweakened strength of signalsince un-conjugated antibody is competing withconjugated antibody. This leads to less conjugated antibody binding to the target and therefore a weaker signal.
I suggest to run an test experiment to see if enough signal can still be obtained and to avoid freeze/thaw cycles at all cost.
Should there be precipitate after thawing, I recommend to spin the vial down and only use the supernatant for experiments
I hope this information helps. Please do not hesitate to contact us if you need anything further.
Answered on Feb 24 2012