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I am inquiring to see if there have been any changes to the Anti-C1q antibody (HRP) product # ab46191. I have been using various lots that have a storage condition of 4+C and state no freezing with wonderful results ( Average Mean OD at my top concentration is 1.8) and I have received a new lot at a 1:200x dilution) and my OD have significantly decreased to ˜ 0.5. Have there been any other issues with this lot of HRP?
Asked on Aug 09 2012
Thank you for contacting Abcam regarding ab46191.
I have looked into the various lots you have used and there have been no changes in the formulations, concentrations, or QC results. Because this is a polyclonal antibody, some lot-to-lot variation is expected and you may need to optimize the dilutions you are working with. I also do have another lot available and can send this if further troubleshooting does not improve your results.
I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions or concerns.
Answered on Aug 09 2012